The fate of pyruvate dictates cell growth by modulating cellular redox potential.

ABSTRACT: Pyruvate occupies a central node in carbohydrate metabolism such that how it is produced and consumed can optimize a cell for energy production or biosynthetic capacity. This has been primarily studied in proliferating cells, but observations from the post-mitotic Drosophila fat body led us to hypothesize that pyruvate fate might dictate the rapid cell growth observed in this organ during development. Indeed, we demonstrate that augmented mitochondrial pyruvate import prevented cell growth in fat body cells in vivo as well as in cultured mammalian hepatocytes and human hepatocyte-derived cells in vitro. We hypothesize that this effect on cell size was caused by an increase in the NADH/NAD⁺ ratio, which rewired metabolism toward gluconeogenesis and suppressed the biomass-s

SUBMITTER: Toshniwal AG 

PROVIDER: S-EPMC12707817 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature