Rapid mitochondrial repolarization upon reperfusion after cardiac ischemia.

ABSTRACT: The mitochondrial membrane potential (ΔΨ_m) drives oxidative phosphorylation and alterations contribute to cardiac pathologies, but real-time assessment of ΔΨ_m has not been possible. Here we describe noninvasive measurements using mitochondrial heme b_L and b_H absorbances, which rapidly respond to ΔΨ_m. Multi-wavelength absorbance spectroscopy enabled their continuous monitoring in isolated mitochondria and the perfused heart. Calibration of heme b absorbance in isolated mitochondria revealed that reduced heme b_L relative to total reduced heme b (fb_L = b_L/(b_L + b_H)) exhibits a sigmoidal relationship with ΔΨ_m. Extrapolating this relationship to the heart enabled estimation o

SUBMITTER: Giles AV 

PROVIDER: S-EPMC12708349 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature