Transaminase-catalyzed sequential hydrodefluorinations of perfluorinated methylene groups.

ABSTRACT: Biocatalysis represents a particularly promising approach for the degradation of recalcitrant fluorochemicals. However, the enzymatic activation of perfluorinated methylene groups remains a challenge, primarily due to the increase in C-F bond strength that correlates with the number of fluorine atoms attached to a single carbon atom. Here, we describe a new-to-nature hydrodefluorination reaction catalyzed by engineered transaminases for the biodegradation of difluorinated fused-ring ketones. Remarkable defluorination efficiency was achieved in most cases by employing a slight excess of 2-PrNH₂ as amine donor. Experimental results provide conclusive evidence that the catalytic process commences with a reversible transamination step. The comprehensive examination of this promiscuo

SUBMITTER: Chen X 

PROVIDER: S-EPMC12708621 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature