Nanopore based RNA methylation profiling of a circulating lung cancer biomarker.

ABSTRACT:

Background

The characterisation of circulating nucleic acid biomarkers in liquid biopsy based diagnostics holds great potential to transform the landscape of early cancer detection and screening. These tests are increasingly incorporating information beyond the primary sequence, to include epigenetic, fragmentomic, and other chemical properties to boost performance. Chemical modifications of RNA offer a rich, and currently underutilised source of biomarker signal. We aimed to develop a single-molecule method to profile 2'-O-methylation of a ribosomal RNA fragment previously linked to lung cancer, and to evaluate its diagnostic value in blood.

Methods

We have designed a targeted capture strategy that ligates structure-guided adapters to a ~22-nucleotide ribosomal RNA fragment

SUBMITTER: Sanchez-Delgado M 

PROVIDER: S-EPMC12708728 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature