Nascent RNA profiling reveals genome-wide productive reiterative initiation regulating gene transcription in bacteria.

ABSTRACT: Productive reiterative initiation at promoters is an alternative transcription mechanism characterized by RNA slippage relative to the template DNA and RNA polymerase, resulting in the incorporation of extra nucleotides into transcripts. A comprehensive understanding of the mechanisms underlying productive reiterative initiation, and its functional implications has been hindered due to the complexity of heterogeneous slippage transcripts. Here, we develop and employ 5' Terminal Native Elongating Transcript Sequencing (5'TNET-seq) to identify and quantify productive reiterative initiation events in Escherichia coli. Using this method, we reveal that more than half of promoters exhibit productive reiterative initiation. The conserved promoter -10 region, an appropriate spacer between the -10

SUBMITTER: Sun Z 

PROVIDER: S-EPMC12709180 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature