Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria.

ABSTRACT: Bacterial transcription regulation is critical for adaptation and survival. CarD is an essential transcription factor in mycobacteria involved in the regulation of gene expression. We searched for CarD interaction partners in Mycobacterium smegmatis and identified a novel uncharacterized protein, named CrsL (MSMEG_5890). CrsL is a 5.7 kDa protein shown by NMR to be intrinsically disordered. CrsL homologs are present in actinobacteria, including pathogenic species such as Mycobacterium tuberculosis. CrsL interacts directly with CarD, adopting an ordered structure in the complex, and also binds RNAP, controlling CarD-RNAP association. ChIP-seq showed that CrsL associates with the promoters of actively transcribed genes and ∼75% of these regions are also associated with CarD. RNA-seq revealed

SUBMITTER: Shoman M 

PROVIDER: S-EPMC12709188 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature