Super-resolution algorithms for imaging FCS enhancement: A comparative study.

ABSTRACT: Understanding the structure and dynamics of biological systems is often limited by the trade-off between spatial and temporal resolution. Imaging fluorescence correlation spectroscopy (ImFCS) is a powerful technique for capturing molecular dynamics with high temporal precision but remains diffraction limited. This constraint poses challenges for quantifying dynamics of subcellular structures like membrane-proximal cortical actin fibers. Computational super-resolution microscopy (CSRM) presents an accessible strategy for enhancing spatial resolution without specialized instrumentation, enabling compatibility with ImFCS. In this study, we evaluated various CSRM techniques, including super-resolution radial fluctuations, mean-shift super-resolution, and multiple signal classification imaging,

SUBMITTER: Pandey S 

PROVIDER: S-EPMC12709258 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature