Anomalous photophysical behaviors attributed to excitonic coupling in fluorescent proteins.

ABSTRACT: Fluorescent proteins (FPs) comprise hundreds of different genetically encoded biosensors. Anomalous FP photo-physics, consistent with excitonic coupling, i.e., delocalized excitation, has been previously reported. Since delocalized excitation can potentially alter fluorescence lifetimes, intensities, and spectra at long distances, its impact needs to be evaluated for the proper design and interpretation of biosensor experiments, as well as for the development of genetically encoded excitonic materials. In addition, it is unclear if excitonic coupling is a shared trait of all β-barrel FPs, nor are the distances requirements for FP excitonic coupling known. To address these questions, we engineered FP constructs having either one or two functional chromophores. We found red shifts in absorpt

SUBMITTER: Nguyen T 

PROVIDER: S-EPMC12709413 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature