Phenotypic and functional characterization of tumor-reactive T cells in malignant pleural effusions.

ABSTRACT:

Background

Adoptive cell therapy using tumor-infiltrating lymphocytes (TIL) is approved for the treatment of advanced melanoma but is limited by the need for patients to undergo surgical tumor resection. Malignant pleural effusions (MPE) may represent a more accessible source of tumor-reactive T cells. Here, we characterize the cellular composition as well as the transcriptional and functional properties of T cells from MPE compared with pulmonary metastasis and blood from a patient with melanoma.

Methods

The immune cellular composition was immunophenotyped by high-dimensional flow cytometry from synchronously collected MPE, a lung metastasis, and blood from a patient with metastatic melanoma. Sorted CD3⁺ T cells were profiled by single-cell RNA sequencing (scRNA-

SUBMITTER: Gaiger NS 

PROVIDER: S-EPMC12709480 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature