Automated GMP-compatible production of universal CAR Tregs for organ-targeted tolerance induction.

ABSTRACT:

Background

Adoptive transfer of regulatory T cells (Tregs) has demonstrated safety, feasibility and early signs of efficacy in promoting immunological tolerance in inflammatory conditions such as graft-versus-host disease (GvHD). Chimeric antigen receptor (CAR)-engineered Tregs offer localized activation and suppression compared to polyclonal Tregs, but their clinical translation is limited by high manufacturing costs, lengthy developing times and fixed single-antigen specificity. To address these limitations, we employed the universal adapter Reverse CAR (RevCAR) system, which harbors a peptide epitope lacking intrinsic antigen specificity but provides flexibility in targeting through the use of an antigen-specific RevCAR Target Module (RevTM). As a proof-of-concept, we used a Rev

SUBMITTER: Lakshmi K 

PROVIDER: S-EPMC12709696 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature