MiST: rapid, accurate and flexible (core-genome) multi-locus sequence typing (MLST) allele calling from draft genomes.

ABSTRACT:

Background

Multi-locus sequence typing (MLST) is a typing method to differentiate bacteria based on the sequence of several housekeeping genes. Identifiers are assigned to unique allele sequences for each locus in the scheme, and the combination of these identifiers defines a genetic profile. Its reproducibility and portability across laboratories have made it a staple typing method, widely used for epidemiological and evolutionary analyses. Although MLST was traditionally based on Sanger sequencing, the method is still widely used in the era of whole-genome sequencing (WGS). Moreover, WGS has made core-genome MLST (cgMLST) possible, which scale up MLST to hundreds or thousands of loci across the genome. Conventional and cgMLST schemes are publicly available on various platforms su

SUBMITTER: Bogaerts B 

PROVIDER: S-EPMC12709719 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature