DTMUV upregulates DDX17 expression to facilitate viral replication.

ABSTRACT: Duck Tembusu virus (DTMUV) is a single-stranded positive-sense RNA virus that poses a serious threat to the poultry industry, and its infection mechanism remains incompletely understood. This study aimed to investigate the role of the host protein DDX17 (DEAD-box RNA helicase 17) in DTMUV infection. Utilizing an in vitro infection model combined with CRISPR/Cas9 gene knockout, siRNA interference, and overexpression techniques, we found that DTMUV infection significantly upregulated both mRNA and protein expression levels of DDX17 in host cells. Furthermore, DDX17 facilitated viral replication through its ATP-binding and hydrolysis domain. Co-immunoprecipitation and mass spectrometry analysis confirmed an interaction between DDX17 and the DTMUV C protein. Additionally, phylogenetic analysis

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC12709809 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature