IMP²RIS, an automated plant root PET radiotracer gas delivery system for in-soil visualization of symbiotic N₂ fixation in nodulated roots of soybean plants via PET imaging.

ABSTRACT: The real-time and non-invasive visualization and quantification of symbiotic nitrogen fixation (SNF) in nodulated roots of soybean plants using Positron Emission Tomography (PET) imaging, coupled with the application of [¹³N]N₂ gas as a PET radiotracer, has been explored in only a few studies. In these studies, [¹³N]N₂ was delivered to nodulated soybean roots suspended in air within gas-tight acrylic boxes, followed by two-dimensional (2D) PET imaging to visualize the assimilated [¹³N]N₂ in the air-suspended root nodules. In this paper, we introduce the In-Media Plant PET Root Imaging System (IMP²RIS), a novel gas delivery system designed and constructed in-house. Unlike the previous methods, IMP²RIS al

SUBMITTER: Nakhforoosh A 

PROVIDER: S-EPMC12709944 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature