Pre-symptomatic diagnosis of rice blast and brown spot diseases using chlorophyll fluorescence imaging.

ABSTRACT: Rice blast and brown spot are two of the most significant fungal diseases affecting rice production. Although the leaf symptoms of both diseases are distinct, they manifest as similar brown-black lesions, complicating differentiation and effective management. Traditional diagnostic methods predominantly rely on DNA-based molecular techniques, which are not well-suited for rapid, large-scale applications. This study aimed to identify reliable chlorophyll fluorescence (ChlF) indicators for diagnosing these diseases at the pre-symptomatic stage using a pulse-amplitude modulation fluorometer. Changes in ChlF parameters were measured following fungal infection in 120 leaves and 750 spots across five time points in detached leaf assays. Diagnostic indicators were selected through machine learnin

SUBMITTER: Lee H 

PROVIDER: S-EPMC12710010 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature