Enhanced vitamin A production by engineering transporters, ATP and precursor supply.

ABSTRACT: Vitamin A (retinoids) is essential for human metabolism and has extensive applications in medicine, health, and cosmetics. Microbial cell factories have been developed for retinoid biosynthesis, coupled with two-phase fermentation for in situ extraction. Given that notable portions of retinoids remained in the cells, promotion of retinoid secretion is expected for further production improvements. This study investigates the potential of yeast endogenous PDR family proteins to enhance retinoid efflux by overexpressing them in retinoid-producing Saccharomyces cerevisiae strains. Among the PDR proteins tested, the transcriptional factor Pdr3p and the transporters Pdr10p and Snq2p significantly enhanced retinol and retinal secretion and production, while the transcriptional facto

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12710018 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature