Development and validation of enzyme-linked immunosorbent assays for the serodiagnosis of canine bartonelloses.

ABSTRACT: Bartonella species, emerging vector-borne pathogens of dogs, are increasingly associated with severe, chronic sequelae, as well as potentially life-threatening diseases, such as endocarditis and myocarditis. Diagnosis of bartonelloses is mainly based on PCR, culture, and serological assays. Despite molecular and biotechnological advances, serological assays employing immunofluorescence antibody (IFA), Western blotting, and enzyme-linked immunosorbent assay (ELISA) technologies have encountered diagnostic limitations, primarily due to poor sensitivity. Using sera from Bartonella-infected and naïve dogs, we applied an immunoproteomic approach to develop a reliable ELISA assay for the diagnosis of bartonelloses in dogs. Five recombinant Bartonella henselae immunodominant

SUBMITTER: Neupane P 

PROVIDER: S-EPMC12710310 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature