Contrasting glucosinolate profiles in rapeseed genotypes shape the rhizosphere-insect continuum and microbial detoxification potential in a root herbivore.

ABSTRACT: Plant secondary metabolites are key mediators of plant-insect-microbiome interactions, yet their role in structuring functionally relevant insect-associated microbial communities remains poorly understood. Here, we combined a factorial experiment using Brassica napus genotypes differing in glucosinolate (GLS) content with distinct succession to investigate the eco-evolutionary dynamics of the microbiota of the root herbivore Delia radicum. Amplicon sequencing and microbial culturing revealed that both rhizospheric and gut microbial communities are shaped by plant genotype and soil legacy, with a subset of bacterial taxa shared across compartments. Notably, Pseudomonas brassicacearum, harboring the isothiocyanates (ITC) detoxifying gene saxA, was consistently rec

SUBMITTER: Carpentier J 

PROVIDER: S-EPMC12710311 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature