Molecular diagnostics in cancer patients with suspected respiratory mold infections.

ABSTRACT: Invasive fungal infections (IFIs), including aspergillosis and mucormycosis, are serious complications in cancer patients. Reliable detection of etiologic agents is challenging but important for optimal patient management. In a prospective multicenter study, we compared fungal detection by culture, specific and unspecific qPCRs, and fluorescence in situ hybridization (FISH) targeting Aspergillus and Mucorales in bronchoalveolar lavage fluid (BALF) and serum of adult cancer patients with suspected fungal pneumonia. In subgroups of patients, fungal DNA was amplified from serum. We evaluated 210 IFI episodes (proven: 3, probable: 72, possible: 107, unclassifiable: 28). Broad-range fungal PCR from BALF was terminated early due to frequent amplification of colonizing yeasts. Speci

SUBMITTER: Rickerts V 

PROVIDER: S-EPMC12710353 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature