Unraveling proteomic chaos by independent component analysis-ClpX proficiency promotes the iron and oxygen limitation responses of <i>Staphylococcus aureus</i> and affects the intracellular bacterial behavior.

ABSTRACT: In the opportunistic pathogen Staphylococcus aureus, protein homeostasis is largely mediated by the caseinolytic protease (Clp) system. The proteases ClpXP and ClpCP are crucial for general and targeted proteolysis, which rely on the unfoldases ClpX and ClpC interacting with specific targets. However, the global effect on the proteome, especially under infection-relevant stresses, is not well understood. To assess the effect of ClpX deficiency during infection-related processes, mass spectrometry-based global proteome profiles of S. aureus HG001 wild type, an isogenic ∆clpX mutant, and a clpX-complemented strain were recorded under control conditions as well as iron and oxygen limitation. The proteomic profiles revealed specific ClpX- and stress-dependent change

SUBMITTER: Busch LM 

PROVIDER: S-EPMC12710355 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature