Role of NK cells in immune escape in patients with classical paroxysmal nocturnal haemoglobinuria.

ABSTRACT:

Background

Paroxysmal nocturnal haemoglobinuria (PNH) is an acquired clonal haematopoietic stem cell disorder. Immune escape is crucial in PNH, and our previous studies revealed that natural killer (NK) cells potential participate in the immune escape of PNH. This study aimed to investigate the subtypes and functional changes of NK cells in PNH patients.

Methods

We analysed CD59⁺ and CD59^- bone marrow mononuclear cells using single-cell RNA sequencing (scRNA-seq). The results were validated through flow cytometry and co-culture experiments.

Results

We classified NK cells into seven subtypes by scRNA-seq, and found significant differences in the distribution of subtypes in CD59⁺ and CD59^- NK cell of PNH patients. Compared wit

SUBMITTER: Wang C 

PROVIDER: S-EPMC12710432 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature