Zinc-Coordinated Trienzyme Nanogel Cascade Therapy for Accelerated Post-Pancreatectomy Cutaneous Wound Healing.

ABSTRACT: Orchestration of enzyme cascades in synthetic systems remains a major challenge for catalytic control in complex biological environments. Here, a zinc-coordinated tri-enzyme nanogel system (Zn@nGSC) is reported that mimics natural enzymatic assemblies by confining individual glucose oxidase (GOX), superoxide dismutase (SOD), and catalase (CAT) within an imidazole-functionalized polymeric nanogel matrix. The nanogel is fabricated via mild in situ polymerization combined with Zn²⁺-imidazole coordination, yielding structurally stable multi-enzyme assemblies. The engineered assemblies demonstrate simultaneous preservation of enzymatic activity and enhanced cascade efficiency under thermal and proteolytic stress. Cascade reactions proceed as follows: i) glucose is depleted by GOX, ii

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12710564 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature