Programmable cell differentiation in budding yeast uncouples reproductive and metabolic tasks.

ABSTRACT: High-yield biomanufacturing requires large cell populations and a mechanism for directing metabolic resources towards product synthesis. However, the resources that support population growth are the same as those that drive productivity, creating a conflict that limits production yields. To overcome this fundamental limitation, we apply the principle of division of labor to separate reproductive and metabolic tasks into distinct cell types within an isogenic Saccharomyces cerevisiae culture. We introduce MiSTY (Microbial Stem Cell Technology in budding Yeast), a genetic platform that exploits natural asymmetric cues to control cell differentiation. Leveraging bud cell-specific transcription, a sequential series of recombinase-based genetic circuits generates Activated Stem Cells (ASCs) tha

SUBMITTER: Guidry J 

PROVIDER: S-EPMC12710643 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature