Astrocytic CREB regulates transcriptomic, neuronal, and behavioral responses to cocaine.

ABSTRACT: Emerging evidence increasingly indicates that astrocytes are involved in neuropsychiatric disorders, including addiction, but the cocaine-induced astrocyte transcriptome has yet to be investigated. We performed RNA sequencing on whole-cell sorted astrocytes from the nucleus accumbens and bioinformatically characterized the astrocyte transcriptome following cocaine self-administration in male mice. We found that astrocytes exhibit robust and contextually specific transcriptional signatures that implicate adenosine 3',5'-monophosphate response element-binding protein (CREB) as a cocaine-induced transcriptional regulator in astrocytes. Cleavage under targets and release using nuclease sequencing (CUT&RUN) revealed increased astrocytic CREB DNA binding in response to cocaine. Viral-mediated ma

SUBMITTER: Holt LM 

PROVIDER: S-EPMC12710716 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature