A Mouse Photoreceptor Proteome Resource Identifies PALS2/MPP6 as a Novel Pan-Cone Photoreceptor Marker.

ABSTRACT:

Purpose

To generate a quantitative proteome expression resource of rod and cone photoreceptors from the mouse retina that allows the exploration of cell type-specific proteomic landscapes.

Methods

Fluorescence-activated cell sorting of rod and cone photoreceptors was followed by in-solution digestion with trypsin using filter-aided sample preparation. Mass spectrometry analysis was performed using data-independent acquisition (DIA).

Results

Demonstrating the potential of our proteome resource, we identified PALS2 (protein associated with LIN7 2), also known as MPP6 (membrane palmitoylated protein 6), as a protein present in cone photoreceptors but absent in rod photoreceptors. We established PALS2/MPP6 as a novel pan-cone photoreceptor marker. Importantly, the high pr

SUBMITTER: Lux UT 

PROVIDER: S-EPMC12710781 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature