Quantitative proteomic and phenotypic responses of urinary pathogens to CuO/Cu₂O nanoparticles.

ABSTRACT:

Aims

To evaluate the antibacterial efficacy of pulsed laser ablation-generated copper oxide (CuO/Cu₂O) nanoparticles (NPs) against urinary pathogens and to elucidate molecular stress responses through proteomic profiling.

Methods

Enterococcus faecalis, Proteus mirabilis, Escherichia coli, and Pseudomonas aeruginosa were exposed to CuO/Cu₂O NPs. Viability was assessed by colony-forming unit counts, while morphological alterations were examined using field emission scanning electron microscopy (FESEM). Quantitative proteomic analysis with COG and KEGG bioinformatics was performed for E. faecalis and P. mirabilis at early exposure times (10-60 min).

Results

CuO/Cu₂O NPs significantly reduced viability, with E. coli and

SUBMITTER: Sung K 

PROVIDER: S-EPMC12710891 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature