Targeted dual selection to optimize transposon stable pool generation of multispecifics.

ABSTRACT: Growing knowledge around disease states has led to opportunities within research to make designer molecules with improved specificity and broader efficacy. These next-generation molecules frequently take advantage of multispecific targeting and controlled mechanisms of action by utilizing four unique peptide chains as seen in many bispecific or trispecific antibodies. However, with all the opportunities these multispecifics offer, their increased biological complexities come with increased challenges during expression and purification to produce high-quality material. Lower yields accompanied with a high degree of mispairing after the initial capture purification step are often limiting factors. Developing new methods for stable pool expression can offer a strong advantage for progressing

SUBMITTER: Johnston J 

PROVIDER: S-EPMC12710902 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature