Top-down enrichment of oil-degrading microbial consortia reveals functional streamlining and novel degraders.

ABSTRACT:

Introduction

Top-down microbial enrichment is a reliable approach for understanding and designing microbiomes for crude oil remediation. Environmental variables serve as valuable determinants for selecting desired microbiomes with superior performance. However, the linkages between selection methods and the structure and function of desired microbiomes remain unclear.

Methods

This study integrated substrate concentration gradients and cultivation patterns to investigate how selection pressures shape top-down enriched crude oil-degrading consortia. The resulting communities were analyzed using 16S rRNA gene sequencing, metagenomics, and co-occurrence network analysis. Key bacterial strains were isolated to validate their individual degradation capabilities.

Results

The

SUBMITTER: Wu H 

PROVIDER: S-EPMC12711144 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature