IS<i>1216</i> drives the evolution of pRUM-like multidrug resistance plasmids in <i>Enterococcus faecium</i>.

ABSTRACT: pRUM-like plasmids are commonly found in multidrug-resistant Enterococcus faecium, but the evolution of these plasmids has not been characterised in detail. When we analysed the genome sequences of two clinical E. faecium strains isolated in Birmingham, UK, we found two pRUM-like plasmids, pHHEf1 and pHHEf2. They were ~25 kb in size and shared the same 10 kb backbone but contained starkly different accessory regions that were bounded by and interspersed with the IS26 family insertion sequence IS1216. pHHEf1 contained a complete set of vancomycin resistance genes, while pHHEf2 contained aminoglycoside and erythromycin resistance genes along with an integrated small plasmid, pCOLA. It appeared that IS1216 had driven the diversification of these accessory re

SUBMITTER: Allen F 

PROVIDER: S-EPMC12711152 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature