High-Throughput Metabolomics using 96-plex Isotope Tagging.

ABSTRACT: Liquid chromatography-mass spectrometry (LC-MS) based metabolomics suffers from extended duty cycles and matrix-dependent quantitation. Chemical tags with 96 unique masses are reported, which alleviate the metabolomic workflow bottleneck and allow for absolute quantitation. A metabolic screen for carboxylic acids was performed on mammalian cells deprived of various nutrients and showed 24% RSD and analysis of 288 samples in 2 h.

SUBMITTER: Armbruster MR 

PROVIDER: S-EPMC12711192 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature