Sex influences murine T cell responses to vaccination with BCG or BCG∆BCG1419 grown as biofilms.

ABSTRACT:

Background

It is known that host sex can influence the immune response to administration of Mycobacterium bovis Bacillus Calmette-Guérin (BCG). However, the effect of BCG or BCG-derived vaccines cultured as biofilms on development of T cell responses in both sexes remains unclear.

Objectives

To compare the influence of sex and vaccine strain (BCG Pasteur vs. BCGΔBCG1419c) on ex vivo T cell responses against mycobacterial purified protein derivative (PPD) stimulation in lung and spleen cells of mice vaccinated with bacteria grown as biofilms.

Methods

Male and female BALB/c mice were subcutaneously vaccinated with disaggregated, biofilm-derived BCG Pasteur or BCGΔBCG1419c. Sixty days later, lung and spleen cells were collected and stimulated ex vivo with PPD. Flow cytom

SUBMITTER: Flores-Valdez MA 

PROVIDER: S-EPMC12711205 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature