Native Mass Spectrometry from Common Buffers with Salts That Mimic the Extracellular Environment.

ABSTRACT: Nonvolatile salts are essential for the structures and functions of many proteins and protein complexes but can severely degrade performance of native mass spectrometry by adducting to protein and protein complex ions, thereby reducing sensitivity and mass measuring accuracy. Small nanoelectrospray emitters are used to form protein and protein complex ions directly from high-ionic-strength (>150 mm) nonvolatile buffers with salts that mimic the extracellular environment. Charge-state distributions are not obtained for proteins and protein complexes from six commonly used nonvolatile buffers and ≥150 mm Na⁺ with conventionally sized nanoelectrospray emitter tips but are resolved with 0.5 μm tips. This method enables mass measurements of proteins and protein complexes directly fro

SUBMITTER: Susa AC 

PROVIDER: S-EPMC12711215 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature