MiR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting <i>LIN28B</i>.

ABSTRACT: MicroRNAs (miRNAs) are emerging as crucial regulators of skeletal muscle development and regeneration; however, the biological functions of many miRNAs remain to be elucidated. In this study, we focused on the function of miR-379-5p, a miRNA we previously identified as highly expressed in the longissimus dorsi muscle of goats. Overexpression of miR-379-5p inhibited the proliferation and differentiation of goat skeletal muscle satellite cells (MuSCs), as evidenced by decreased expression of proliferation and differentiation markers, reduced EdU⁺ cells, and lower myotube formation. Through bioinformatics prediction and experimental validation, we identified LIN28B as a direct downstream target of miR-379-5p. Functional assays revealed that LIN28B promoted the prolife

SUBMITTER: Hou W 

PROVIDER: S-EPMC12711551 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature