Florigen activation complex forms via multifaceted assembly in Arabidopsis.

ABSTRACT: Florigen, encoded by FT genes, is synthesized in leaves and transported to the shoot apical meristem (SAM) to induce flower development^1-3. At the SAM, 14-3-3 proteins are proposed to act as receptors for FT protein and to mediate the indirect interaction between FT and the basic leucine zipper (bZIP) transcription factor FD to form the florigen activation complex (FAC) that activates transcription of flowering genes^4-6. Here we demonstrate a different mechanism of FAC assembly, diverse functions for the 14-3-3 proteins within the complex, and an unexpected spatiotemporal distribution of the FAC. We show that FT is not recruited by 14-3-3 alone, but that it interacts with the DNA-FD-14-3-3 complex through two interfaces, one of which binds DNA via the unstructured C t

SUBMITTER: Gao H 

PROVIDER: S-EPMC12711580 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature