Multi-omic profiling reveals age-related immune dynamics in healthy adults.

ABSTRACT: The generation and maintenance of immunity is a dynamic process that is dependent on age^1-3. Here, to better understand its progression, we profiled peripheral immunity in more than 300 healthy adults (25 to 90 years of age) using single-cell RNA sequencing, proteomics and flow cytometry, following 96 adults longitudinally across 2 years with seasonal influenza vaccination. The resulting resource generated a single-cell RNA-sequencing dataset of more than 16 million peripheral blood mononuclear cells with 71 immune cell subsets from our Human Immune Health Atlas and enabled us to interrogate how immune cell composition and states shift with age, chronic viral infection and vaccination. From these data, we demonstrate robust, non-linear transcriptional reprogramming in T cell sub

SUBMITTER: Gong Q 

PROVIDER: S-EPMC12711581 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature