Age-(in)dependent altered molecular mechanisms in Parkinson's disease through extracellular vesicle proteome and lipidome.

ABSTRACT: Parkinson's disease (PD) remains insidious and clinically elusive at early stages due to the lack of precise, non-invasive biomarkers. Given their ability to cross the blood-brain barrier, extracellular vesicles (EVs) offer a promising platform for biomarker discovery in neurodegeneration. Using an affinity-based EV isolation method, we profile EV proteomes and lipidomes from plasma across life stages, followed by targeted validation via parallel reaction monitoring (PRM). We identify both age-independent and age-dependent EV biomarkers predictive of prodromal PD and capable of distinguishing PD from multiple system atrophy (MSA). Functional analyses reveal protein-lipid interactions contributing to PD pathophysiology, including lipid-mediated modulation of APOE signaling. Machine learning

SUBMITTER: Zhang YT 

PROVIDER: S-EPMC12711695 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature