A data-driven model of macrophage polarization states reveals an IFN macrophage signature in active Crohn's disease.

ABSTRACT: Macrophages are key innate immune cells responsible for initiating and coordinating immune responses. A major determinant of macrophage function is their tissue-context-dependent polarization state, regulated by the cytokines present in the tissue microenvironment. Yet, the capability of characterizing macrophage polarization states in clinical studies remains limited. Here, we used a defined set of cytokines to polarize human PBMC-derived macrophages and determine their transcriptional signatures and stimulus responsiveness. The resultant atlas of transcriptional signatures for human macrophage polarization states was applied to a dataset of intestinal biopsies from Crohn's disease patients and healthy controls. Our analysis identified a dominant population of IFNγ-polarized macrophages i

SUBMITTER: Lowe SC 

PROVIDER: S-EPMC12711780 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature