ClpB affects biofilm formation in methicillin-resistant <i>Staphylococcus aureus</i>.

ABSTRACT:

Introduction

This study aims to explore the effects of the molecular chaperone ClpB on the biofilm formation and pathogenicity of methicillin-resistant Staphylococcus aureus (MRSA).

Methods

The biological membrane formation was evaluated by constructing a clpB knockout strain (ΔclpB) and a complemented strain (CΔclpB) of USA300 MRSA, followed by crystal violet staining, scanning electron microscopy, confocal laser scanning microscopy, and quantitative analysis of extracellular matrix components. A mouse skin infection model was subsequently employed to assess wound healing, histopathological changes, and the expression levels of inflammatory factors.

Results

The results showed that compared with the wild strain (WT), the biomass of ΔclpB

SUBMITTER: Yang M 

PROVIDER: S-EPMC12711867 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature