Impact of different culture media and drying treatment on the isolation of Melissococcus plutonius, the causative agent of European foulbrood, from honey bee larval specimens.

ABSTRACT: Melissococcus plutonius, the bacterium causing European foulbrood in honey bees, includes two distinct types: typical strains, which are fastidious and not easy to culture, and atypical strains, which grow more robustly and tolerate higher sodium levels. Several culture media have been used to isolate this pathogen, and growth dynamics and survival vary according to strain type and medium. The most commonly used media differs across countries, as does the ratio of typical to atypical strains isolated, implying that the choice of medium influences the type of M. plutonius that is more likely to be recovered. This study examined how two commonly used media, Basal and KSBHI, affect the isolation of typical and atypical strains from larval samples co-infected with both types. Both media suppor

SUBMITTER: Kitamura Y 

PROVIDER: S-EPMC12712217 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature