Arabidopsis root lipid droplets are hubs for membrane homeostasis under heat stress, and triterpenoid synthesis and storage.

ABSTRACT: Plant lipid droplets (LDs) and their associated proteins have numerous subcellular and physiological functions. While considerable progress has been made for LDs in many tissues, the function and composition of LDs in roots remain largely unexplored. We investigated the changes in the number of LDs and the lipidome in heat-stressed Arabidopsis thaliana roots. Furthermore, we isolated root LDs from the Arabidopsis mutant trigalactosyldiacylglycerol 1-1 sugar dependent 1-4 and investigated its proteome and lipidome. Heat stress led to a degradation of membrane lipids and an increase in triacylglycerols and LDs, while fatty acid steryl esters decreased, probably acting as precursors for acylated sterol glycosides. A variety of proteins were enriched in root LDs, which are thus far not describ

SUBMITTER: Scholz P 

PROVIDER: S-EPMC12712439 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature