Amide-Linked Local Anesthetics Alter Tumor Biology in a Complex Human Tissue Model of Non-Small Cell Lung Adenocarcinoma.

ABSTRACT: Amide local anesthetics (LA) affect tumor burden in various preclinical studies, possibly via their anti-inflammatory properties. However, a translation into clinical evidence is still lacking. Here, effects of LA at clinically relevant concentrations are assessed using a human ex vivo tumor model of patient-derived tumor slice cultures from nine patients with non-small cell lung cancer. Tumors are cultivated for four days and treated with LA in absence/presence of cisplatin. Tumor cell proliferation and apoptosis as well as expression of intercellular adhesion molecule-1 and macrophage polarization are assessed using immunofluorescent imaging. Tumor specimens are considered to be "Responders", when a change in proliferation and/or apoptosis by >50% compared to untreated slices occurred. F

SUBMITTER: Kromer J 

PROVIDER: S-EPMC12712772 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature