Channel-specific differential effects of bacterial mechanosensitive channels for ultrasound neuromodulation in precision sonogenetics.

ABSTRACT: Rationale: Ultrasound neuromodulation offers promising therapeutic potential, but its effectiveness is limited by imprecise targeting of neural circuits. Engineering mechanosensitive ion channels can enhance ultrasound sensitivity, providing a more precise approach for targeted neuromodulation. This study aimed to compare three bacterial mechanosensitive channels (MscL-G22S, MscL-G22N, and MscS) for mediating ultrasound-responsive hippocampal activity to identify optimal candidates for precision sonogenetics applications. Methods: We expressed MscL-G22S, MscL-G22N, and MscS in the rat hippocampus using AAV vectors and applied focused ultrasound stimulation at various intensities while recording local field potentials. Neural oscillatory patterns, ultrasound-evoked potentials,

SUBMITTER: Li X 

PROVIDER: S-EPMC12712913 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature