A Field-Deployable RotEx-LAMP-LFA Platform for Molecular Triage of HPV-Driven Oncogenesis.

ABSTRACT: Persisting as a major global health disparity, HPV16/18-associated cervical carcinogenesis continues to disproportionately affect populations in resource-limited regions with compromised screening infrastructure. While molecular detection of E6/E7 oncogenic transcripts surpasses conventional cytology in clinical specificity, current nucleic acid amplification platforms remain impeded by technical complexity, prolonged turnaround times (>4 h), and substantial per-test costs (>$50) that hinder scale-up in low-income settings. Here, RotEx-LAMP-LFA is presented: an innovative point-of-care system integrating microfluidic nucleic acid extraction, rapid isothermal amplification (30 min at 63 °C), and combined lateral flow detection in a single disposable cartridge. Validated with 69 clinical spe

SUBMITTER: Gao Y 

PROVIDER: S-EPMC12713021 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature