Dual-Pedicle Tissue-Engineered Trachea Promotes Biomimetic Cartilaginous Framework, Vascularization, and Epithelial Lining for Long-Segment Tracheal Reconstruction.

ABSTRACT: Long-segment tracheal reconstruction remains a formidable clinical challenge due to failures of conventional tissue-engineered trachea (TET) grafts, stemming from inadequate vascularization, compromised cartilaginous framework, and epithelial deficiency. Transcriptomic profiling of conventional TETs revealed enriched hypoxia and inflammatory pathways, underscoring the need for robust vascular and epithelial strategies. Herein, a dual-pedicle biomimetic TET is developed to provide a stable cartilaginous framework for airway support, immediate blood supply for tissue perfusion, and functional epithelial lining for barrier protection. Autologous rabbit auricular skin is sutured into tubes for epithelial lining, while chondrocytes in photo-crosslinkable decellularized Wharton's jelly matrix hy

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12713032 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature