Transcriptomics Guided Engineering of Exosome-Encapsulated Bifunctional Nanosheets Targeting the Immune-PI3K/Akt Axis for Osteoporosis Therapy.

ABSTRACT: Osteoporosis, characterized by imbalanced bone metabolism and chronic inflammation, remains a therapeutic challenge due to the limitations of current single-target therapies. This study integrates transcriptomic insights with nanomaterial engineering to develop a multi-target strategy. Transcriptomic analysis of ovariectomized (OVX) mice reveals immune dysregulation and PI3K/Akt pathway activation, driving osteoclastogenesis. To address this, cobalt-aluminum layered double hydroxide nanosheets (f‑CA(OH)) are synthesized, which scavenge reactive oxygen species (ROS) via peroxidase-like activity and stabilize hypoxia-inducible factor 1α (HIF‑1α) to upregulate BMP2 expression. Co-culturing f‑CA(OH) with mesenchymal stem cells (MSCs) generate engineered exosomes (fCA‑BExo), encapsulating BMP2

SUBMITTER: Tang R 

PROVIDER: S-EPMC12713082 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature