Arabidopsis TRB Proteins Form Two Closely Related Complexes to Mediate H3K4me3 Demethylation and Transcriptional Repression.

ABSTRACT: The Arabidopsis telomere repeat binding proteins TRB1, TRB2, and TRB3 (TRB1/2/3) are components of the PWWP-EPCR-ARID-TRB (PEAT) complex, which is involved in histone acetylation and H2A deubiquitination. TRB1/2/3 also interact with the Polycomb Repressive Complex 2 (PRC2), which mediates H3 lysine 27 trimethylation. This study demonstrates that TRB1/2/3 can form two closely related complexes: TRB1/2/3-HELIX-TURN-HELIX-PROTEIN (TRHT) and TRB1/2/3-HISTONE-DEMETHYLASE (TRHD). The TRHT and TRHD (TRHT/TRHD) complexes occupy numerous common target genes across the genome and are required for H3K4me3 demethylation mediated by the histone demethylase JMJ14. Within the TRHT/TRHD complexes, the transcriptional repressors TRB1/2/3 and NAC050/052 bind to distinct DNA motifs and cooperate to mediate t

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12713088 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature