Microglial TIA1-mediated stress granules promote neuroinflammation and aggravate neuron loss in mice after ischemic stroke by inhibiting IGF2 signaling.

ABSTRACT: Rationale: Microglia cells as niche homeostasis monitor with rapid responses to acute ischemic stroke (IS). T-cell intracellular antigen 1 (TIA1), a core component of stress granules (SGs), is involved in cellular stress responses such as hypoxia, but its roles and mechanisms in regulating microglial responses during IS remain unclear. Methods: To evaluate the function of microglial TIA1 in IS, we established a mouse model of IS by using photothrombotic method. Furthermore, conditional knockout (CKO) of Tia1 in microglia mice (Tia1 ^Cx3cr1-CKO mice) was generated and then Tia1 ^Cx3cr1-CKO IS mice and their littermate controls (Tia1 ^f/f IS mice) were used as experimental subjects. The behavioral tests, immunostaining,

SUBMITTER: Qian Y 

PROVIDER: S-EPMC12713190 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature