Intradermal Inoculation of Mycobacterium avium in the Mouse Ear.

ABSTRACT: In contrast to natural nontuberculous mycobacterial (NTM) exposures or infections whose pathology is founded on a slow-replicating organism, the aerosol infection in a mouse model can cause an elevated proliferation of bacterial growth in the lungs. Chronic aerosol and systemic infections can be treated with anti-mycobacterial drugs, which could also reflect a prior exposure, but these procedures are lengthy and less representative of natural exposures. The goal of this protocol is to develop a less invasive mouse model of NTM exposure-like conditions that are more translational. Here, we describe a method to intradermally challenge preclinical mice through ear tissue using Mycobacterium avium (M. avium) to mimic an exposure and enable immune responses to the bacteria without obvious disea

SUBMITTER: Kaur S 

PROVIDER: S-EPMC12713364 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature