Effects of sporulation times in liquid or on plates on <i>Bacillus subtilis</i> spore resistance, germination, inner membrane fluidity and permeability, and core contents.

ABSTRACT: This study aimed to determine reasons that longer sporulation times in liquid or on plates have large effects on Bacillus subtilis spore germination and resistance. B. subtilis spores were prepared for 3/30/60 days in liquid or on plates and their germination, resistance, levels of core water and Ca²⁺-dipicolinic acid (CaDPA), and inner membrane (IM) fluidity and permeability were measured. Liquid spores of 3/30/60 days had no differences in wet heat resistance, while plate spores of 30/60 days had higher wet heat resistance than 3-day plate spores. There were minimal increases in 3- to 60-day liquid spores' resistance to UV radiation and chemicals, while plate spores of 30/60 days had increased resistance to chemicals. Germinant receptor (GR) germination with L-va

SUBMITTER: Ye F 

PROVIDER: S-EPMC12713371 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature