Mutations in ribosomal protein uS5 alter translation fidelity and mutagenesis in <i>Pseudomonas putida</i>.

ABSTRACT: Errors in protein synthesis can influence cellular fitness and, in some cases, affect genome stability. While this connection has been explored, most investigations have focused on Enterobacteriaceae such as Escherichia coli, leaving open the question of how translational fidelity shapes mutagenesis in other bacterial groups. Would a metabolically versatile, stress-tolerant soil bacterium behave differently? We focused on the small-subunit ribosomal protein uS5 (rpsE) because, despite its role in decoding accuracy, its potential as a mutator locus has not been evaluated. To explore this, we isolated several mutants of ribosomal protein uS5 (rpsE) in Pseudomonas putida, all carrying amino acid substitutions or deletions in loop 2, via spectinomycin selection, and

SUBMITTER: Jurgenstein K 

PROVIDER: S-EPMC12713390 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature