Semisynthetic, Multicolor Probes for Bioluminescence Imaging.

ABSTRACT: Bioluminescence imaging is a sensitive and noninvasive technique for monitoring molecular and cellular processes in living systems. NanoLuc is one of the brightest and most widely used luciferase enzymes; however, the native blue emission of NanoLuc limits its applications due to poor sample penetration. NanoBiT, a split luciferase reporter derived from NanoLuc, consists of two subunits: LgBiT and HiBiT. Herein, we leverage bioluminescence resonance energy transfer (BRET) to red-shift the emission of NanoBiT by chemically labeling the N-terminus of HiBiT with fluorescent probes. Upon association of HiBiT and LgBiT, the appended fluorophores emit red-shifted light. When LgBiT is genetically encoded on the surface of mammalian cells, each probe produces unique emission profiles that can be d

SUBMITTER: Tran S 

PROVIDER: S-EPMC12713427 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature